| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Earls, Graham B (11)
|
OHGMVY |
36.82 |
33.86 |
-2.96 |
| 2 |
Yingling, Jack G (12)
|
OHGMVY |
35.29 |
34.20 |
-1.09 |
| 3 |
Novitske, Daxton M (11)
|
OHCFY |
36.13 |
35.65 |
-0.48 |
| 4 |
Soppe, Austin (12)
|
OHHHY |
34.11 |
37.46 |
3.35 |
| 5 |
Coxhead, Daniel J (12)
|
OHGMVY |
43.25 |
38.79 |
-4.46 |
| 6 |
Spence, Evan D (11)
|
OHSPY |
38.55 |
38.98 |
0.43 |
| 7 |
Smith, Samuel K (12)
|
OHKEY |
37.88 |
39.10 |
1.22 |
| 8 |
Gessner, Brock D (11)
|
OHMCY |
41.34 |
43.09 |
1.75 |
| 9 |
DiTommaso, Anthony J (11)
|
OHCFY |
42.11 |
43.15 |
1.04 |
| 10 |
Roberts, Jonas E (11)
|
OHKZY |
42.43 |
44.65 |
2.22 |
| 11 |
Pfeiler, Alexander K (12)
|
OHKZY |
53.82 |
50.43 |
-3.39 |
| 12 |
Suchland, Logan A (11)
|
OHKZY |
1:07.25 |
53.21 |
-14.04 |
| 13 |
Markeson, Ethan D (12)
|
OHMCY |
48.07 |
53.39 |
5.32 |
|
Pfeiler, Javier T (11)
|
OHKZY |
37.30 |
DQ |
- |
|
Sanders, David (11)
|
OHCFY |
50.70 |
NS |
- |